无翅型MMTV整合位点家族成员11(WNT11)重组蛋白Recombinant Wingless Type MMTV Integration Site Family, Member 11 (WNT11)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species | Homo sapiens (Human) |
Product No. | XY552Hu01 |
Source | Prokaryotic expression |
Host | E.coli |
Purity | > 95% |
UOM | 50ug |
无翅型MMTV整合位点家族成员11(WNT11)重组蛋白Predicted Molecular Mass | 20.5kDa |
Concentration | 8.8 |
Applications | SDS-PAGE; WB; ELISA; IP. |
Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
SEQUENCES
无翅型MMTV整合位点家族成员11(WNT11)重组蛋白The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SLEMKCK CHGVSGSCSI RTCWKGLQEL QDVAADLKTR YLSATKVVHR PMGTRKHLVP KDLDIRPVKD SELVYLQSSP DFCMKNEKVG SHGTQDRQCN KTSNGSDSCD LMCCGRGYNP YTDRVVERCH CKYHWCCYVT CRRCERTVE
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
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更新时间:2024/3/23 17:23:38